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 Fundamental to both of the above is the ability to quantitatively monitor the potency of immunotherapy mediated killing of target cells under controlled conditions in vitro. Traditional cell killing assays suffer from drawbacks that prevent them from meeting this need efficiently.Immune cell mediated killing can be studied by measuring the activation of effector cells or their secretion of cytotoxic molecules (perforin, granzymes, etc.). While these readouts are indeed useful for reductionistic biochemical characterization, they don't necessarily correlate with target cell killing efficiency, which is the ultimate measure of therapeutic efficacy. Assays focused on the response of target cells primarily monitor the release of either previously added labels (such as 51Cr or fluorescent dyes) or endogenous biomolecules (GAPDH, LDH, etc.) upon target cell lysis.Besides the potential artifacts associated with using exogenous labels, the time frame over which such labels are useful is e
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